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KMID : 0900919980220010035
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Korean journal of Animal Reproduction
1998 Volume.22 No. 1 p.35 ~ p.42
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Study on Development of In Vitro Culture Medium for Rabbit Embryos
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Lim Kyung-Sun
Jin Dong-Il
Kim Sung-Woo
Choi Hwa-Sik
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Abstract
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This experiment was carried out to improve in vitro development of rabbit one-cell embryos to the blastocyst stage. One-cell rabbit embryos were collected at 19ulcorner20hr after superovulation induction and incubated at 39ulcorner in 5% CO2 for 72hr. In order to find optimum conditions in medium that affects the rabbit embryo’s development in vitro, RDH medium which mixed with RPMI1640, DMEM and Ham’s F10 was compared with the previously reported mediums (Ham’s F10 and RD) for embryo development and cell numbers. Three additives (BSA, taurine and glucose) were tested for the development of rabbit one-cell embryos in vitro. When the embryos were cultured in RDH medium, their development was markedly promoted as compared with Ham’s F-10 or RD alone. Glucose exhibited no significant effects on embryo development and cell numbers. BSA a, pp.ared to promote transition from morula to blastocyst stage and taurine increased cell numbers of cultured embryos markedly regardless of medium. BSA and taurine together in RDH medium showed the additive effects on embryos development and cell number.
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KEYWORD
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In vitro development, Rabbit embryo, Meidum
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